Conference Proceedings · 2022
Low-cost Polymerase Chain Reaction (PCR) Technology for the Detection of DNA Amplicons
Prashant Waiba, Pravin Bhattarai, Mitesh Shrestha, Ashim Dhakal
Presented in International Conference on Bioscience and Biotechnology, Suraha, Nepal 2022
The design of Pagoda PCR is gleaned from the OpenPCR concept. The reliability of PCR results is dependent on how precisely the intended amplicons are amplified during the thermocycling process [1]. In Pagoda PCR, a single Peltier module is used with a commonly available CPU cooler fan to heat and cool the thermal block during heating and cooling cycles, and a separate heating strip on the lid ramps up the temperature to 200°C. A simple desktop interface is developed using HTML and linked to the ESP8266 WIFI module through Arduino IDE so that any computer connected to the device-defined IP address can access the console which enables the user manually set the PCR protocols.